A Novel Technique for Imaging and Analysis of Hair Cells in the Organ of Corti Using Modified Sca/eS and Machine Learning
Here, we describe a sorbitol-based optical clearing method, called modified Sca/eS that can be used to image all hair cells (HCs) in the mouse cochlea.This modification of Sca/eS is defined by three steps: decalcification, de-lipidation, and refractive index matching, which can Stuffed Toy all be completed within 72 h.Furthermore, we established au